PGD - Preimplantation Genetic Diagnosis – The Newest ART
from the book How to Have a Baby:
by Dr. Aniruddha Malpani, MD and Dr. Anjali Malpani, MD.
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PGD, or preimplantation genetic diagnosis, is a new technique, which marries
the recent spectacular advances in molecular genetics and assisted reproductive
technology. Preimplantation genetic diagnosis enables physicians to identify
genetic diseases in the embryo, prior to implantation, before the pregnancy is
established. PGD was first developed for patients who were at risk of having
children with serious genetic disorders, which often discouraged them having
their own biological children. These couples are often faced with attempting a
type of "Russian Roulette" to have children, many times having to
confront the difficult decision to terminate an affected pregnancy. Consider a
woman known to be carrying an X-linked disease with a 50% risk of an affected
male in each pregnancy. In addition, her daughters have a 50% risk of being
carriers, but are unlikely to be clinically affected. She may not wish to become
pregnant if she has to make decisions about an affected child in a viable
pregnancy. However, she would become pregnant if she knew she had conceived a
daughter, and with preimplantation diagnosis this possibility becomes a reality.
PGD thus eliminates the need for possible pregnancy termination after prenatal
diagnosis of a genetically-affected fetus.
Research has shown that it is possible at three days after fertilisation to
remove one or two cells from an 8-10 celled embryo without detriment to its
further development. Embryos were sexed on the basis of the presence or absence
of a DNA fragment specific for the Y chromosome; in 1990 two sets of twin girls
were born to five couples at risk of passing on an X linked disorder.
Subsequently, a number of babies have been born after the preimplantation
genetics has ruled out diagnosis of cystic fibrosis, Tay Sachs disease, Lesch
Nyhan syndrome, Duchenne muscular dystrophy and for diseases carried on the X
Sexing the embryo to avoid X linked disease remains the commonest reason for
preimplantation diagnosis, now optimally carried out by the molecular cyto
genetic technique of FISH (fluorescent in situ hybridisation) with DNA probes
derived from the X and Y chromosomes.
How is PGD done? After IVF, on the 3rd day, the 8-cell embryo is biopsed. to
obtain blastomeres (single cells) for molecular diagnosis. An embryo biopsy is
done using micromanipulators under the visual control provided by an inverted
tissue culture microscope. The embryo is held in position using a holding
pipette, while a glass needle is used to drill a hole through the zona pellucida
(the shell or the outer layer of the embryo ) using a laser or acid Tyrode's. A
single cell is then removed by gentle suction. The cell (called a blastomere) is
then available for genetic diagnosis.
Fig 1. Embryo biopsy, with a single blastomere being
sucked out from the 8-cell embryo. This will be sent for analysis.
Analysis of genetic material (DNA) from a single cell is performed either
using a technique called FISH ( fluorescent in situ hybridisation) or PCR (
polymerase chain reaction) . FISH utilises fluorescent probes, which are
specific for a given chromosome, and therefore allows one to screen embryos for
chromosomal normality. PCR allows one to amplify (mutiply ) a selected DNA
sequence of interest, so that it can be analysed. After the analysis on the
single cell, the embryos are kept in culture and allowed to further divide. Once
the appropriate molecular diagnosis is made, unaffected embryos can be
transferred back into the uterus in the IVF cycle.
PGD is now also being used in order to increase pregnancy rates for older
infertile women. One of the reasons older women have a poorer pregnancy rate is
because their embryos are often chromosomally abnormal, because of the fact they
have older eggs ( which may have genetic defects). PGD allows the doctor to
select only the chromosomally normal embryos, so that only these can be
transferred back into the uterus, resulting in a higher pregnancy rate.
PGD for sex selection – right or wrong
While PGD represents the cutting edge of reproductive technology, and gives
us an idea of what may be possible for the future, it also raises a number of
worries and concerns, especially in India, where people are worried that it may
be used for sex-selection.
PGD is emotionally a very touchy area, because not only are we dealing with
human embryos - the very start of new life, but we are studying their basic
blueprint - their genes - the stuff of which humanity is made. Obviously, this
is likely to cause people to take very strong views on what is right and what is
wrong - so that they start thinking with their hearts rather than their heads !
Many people confuse PGD with genetic engineering. A familiar refrain is we
shouldn't be doing any of this because scientists are becoming too big for their
boots - they are trying to play God by tinkering with the genes , and it is far
better that they leave this entire field well alone, since we will never be able
to understand any of it - it is beyond human wisdom. This is a common knee-jerk
reaction, which precludes further rational debate.
The other view point is - Why not ? If man can improve on Nature, then why
should he not try? After all, building a house is simply man's way of improving
on nature - and if we can improve man himself, then why not? Seen in this light,
then studying the molecular genetics of the human embryo would be the ultimate
goal of all medicine. In the past, doctors used to treat adults. In the
beginning of the 20th century, we started treating children, and the field of
pediatrics was born. We can now treat the fetus - and the future patient of the
21st century will be the embryo - this is a logical progression!
If we allow people to choose when to have babies; how many to have; and even
to terminate pregnancies if they inadvertently get pregnant, then why not allow
them to select the sex of their child, if it is possible?
We should allow patients freedom to choose for themselves - medical
technology should empower them with choices they can make for themselves! A
common criticism against PGD for sex selection is that it will cause an
unbalanced sex ratio. In reality, PGD will allow couples to balance the sex
ratio in their families, rather than unbalance it! For example, take a couple
with a baby girl, who want to have a second baby. If they leave things upto
chance, half of them will have a second baby girl - causing unbalanced
intrafamily sex ratios ! PGD will allow them to make sure that they have a
balanced sex ratio in their family, if they so desire. Seen in this light, PGD
is perhaps the ultimate form of family planning there is!
by Dr. Aniruddha Malpani, MD and Dr. Anjali Malpani, MD.
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